President Donald Trump's administration on Friday ousted the two witnesses who provided the most damaging testimony during his impeachment investigation: Army Lieutenant Colonel Alexander Vindman and Ambassador Gordon Sondland.

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US President Donald Trump speaks to the media after participating in a video teleconference with members of the US military.Reuters

Two days after Trump was acquitted by the Republican-controlled Senate on charges of trying to pressure Ukraine to investigate a political rival, Vindman — the top Ukraine expert at the White House's National Security Council — was escorted out of the building, according to his lawyer.

"Vindman was asked to leave for telling the truth," said his lawyer, David Pressman.

Hours later, Sondland said he had been fired from his post as US ambassador to the European Union.

The two men served as star witnesses during the Democratic-controlled House of Representatives' impeachment investigation last year.

Vindman's twin brother Yevgeny, who worked as a lawyer at the NSC, also was escorted out of the White House, according to Michael Volkov, who represented Vindman when he testified in the impeachment inquiry.

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Trump has said he is still upset with Democrats and government officials involved in the impeachment investigation, even after he was acquitted on Wednesday.

"I'm not happy with him. You think I'm supposed to be happy with him?" he said of Vindman on Friday.

Vindman, a decorated combat veteran, testified in November that he "couldn't believe what I was hearing" when he listened in on a July 25 phone call between Trump and Ukrainian President Volodimir Zelenskiy that became the focus of the inquiry.

Trump asked Zelenskiy to launch investigations into both Democratic rival Joe Biden and a discredited theory that Ukraine, not Russia, colluded with Democrats to harm Trump in the 2016 US presidential election.

Sondland, a wealthy Republican donor and Oregon hotelier who served as U.S. Ambassador to the European Union, testified that he was following Trump's orders when he pushed Ukrainian officials to carry out investigations sought by the president.

"I am grateful to President Trump for having given me the opportunity to serve," he said.

The White House and State Department did not immediately respond to requests for comment on Sondland's removal.

"This is as clear a case of retribution as I've seen during my 27 years in the Senate," said Democratic Senator Dianne Feinstein.

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US President Donald Trump and first lady Melania Trump depart Joint Base Andrews in Maryland.Reuters

Biden's campaign for the Democratic presidential nomination suffered a serious setback when he came in fourth place at the Democrats' first state contest in Iowa this week.

Vindman's two-year stint at the White House had been due to end in July. An Army spokesperson said both brothers had been reassigned to the Army, but declined to give further information "out of respect for their privacy."

Another senior White House aide who testified over impeachment, Jennifer Williams, left this week for a post at the U.S. military's Central Command, according to Bloomberg News.

Vindman downplayed concerns that he would suffer payback for speaking out when he testified to Congress. "I will be fine for telling the truth," he said.