Having already recorded a 2-1 victory at Manchester City in the first leg, Barcelona will be pretty confident of reaching the last eight of the European competition.
Apart from that, they have also been doing well domestically as they current at the top of the La Liga and the final of the Copa del Rey.
The Catalans' record against the defending English champions is also pretty, having managed to beat the Citizens in the both the legs (4-1 aggregate win) at the same stage of the Champions League last season.
However, Barcelona boss Luis Enrique insists that his side's victories over City mean nothing and they still have to be at their very best in the second leg on Wednesday.
"I don't think last season's game is anything to go by because both sides have changed players. You can't qualify just by playing one of the two games very well. We need to play well [in the second leg] too and see if we can beat City again," the 44-year-old told UEFA's official website.
"I don't think City look physically tired at all. I am sure they will fight to qualify for the next round – I'm positive of that. We don't take anything for granted and this match is very important."
Right after this fixture, Barcelona face archrivals Real Madrid in an important La Liga fixture, but Enrique insists that his side are only focusing on their clash against City.
"There's only one match on our minds and that's the one against City. Not the Clásico," he added.
"It would be dangerous to play a cagey match. Our objective is to win and play better than our opponents. A place in the next round is at stake. You play the whole season for these games and that's why the press room is full today."
Meanwhile, City will also be fancying their chances at Barcelona despite them conceding two home goals in the first fixture.
The Citizens have not been in the best of forms of late, having recently recorded a 0-1 defeat at the hands of a struggling Burnley side in the Premier League.
They are certainly not expected to beat Barcelona at the Nou Camp especially taking into account the Catalans' current form.
But City boss Manuel Pellegrini insists his side have a good chance of turning things over in the second leg if they play like the way they did in the second half in the first leg at the Etihad.
"We need to play like we did in the second half of the first leg," the Chilean told UEFA's official website. "For part of the first half Barça controlled the game but in the second half we had chances. We need to play 11 v 11 and then we have a team that can play like we did in the second half."
"It's important to fight Barcelona for the ball but they dominate possession against all opponents. We have to keep the ball once we gain control of it. We want to give Barcelona problems regaining the ball once we have it."
The former Malaga boss feels that it is very "important" for City to beat Barcelona and prove that they are "one of the best teams in Europe."
"It is important for us to prove that we are one of the best teams in Europe by beating Barcelona and that must be our test. We must strive to win."
Barcelona: Ter Stegen; Alves, Piqué, Mascherano, Alba; Xavi, Rakitić, Iniesta; Suárez, Messi, Neymar.
Manchester City: Hart; Zabaleta, Kompany, Demichelis, Kolarov; Fernando, Fernandinho; Milner, Touré, Silva; Agüero.
Where to Watch Live
The game is scheduled for an 8:45pm local time (1:15am IST, 7.45 pm GMT, 3:45pm ET) start, with live coverage on Ten Action and Ten HD in India. The match can also be watched via live streaming online on Ten Sports Live.
Catch the action live in the UK live on Sky Sports 5 and Sky Sports 5 HD while viewers can also watch the watch online via live streaming on Sky GO. US viewers can watch the game live on Fox Sports 1 or online on Fox Soccer 2Go. Audience in Canada can see the tie via live stream on Sportsnet World.
Viewers in the Middle East and North Africa can watch the match via live streaming online on beIN Sports Connect, while South Africa and Sub Saharan Africa viewers can do the same on SuperSport online.